林一郎 助理教授
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姓 名： 林一郎 Lin Elong
職 稱： 助理教授
研究室：保健大樓 3 樓 / 9310室
電 話： 04-22391647轉3519 3533
傳 真： 04-22396771
E-mail： yllin@ctust.edu.tw
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學歷
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國立陽明大學遺傳學研究所博士
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私立中山醫學院營養系學士
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專長
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遺傳暨分子生物學
生化暨蛋白質化學
病毒學
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專長技術
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Biochemistry: Chromatography( HPLC, ion exchange, gel permeation ), Electrophoresis, Two dimension electrophoresis, Sucrose gradient, Glycerol gradient, CsCl gradient, Gel shift( electrophoresis mobility shift assay; EMSA ), Radiochemistry and Protein purification.
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Molecular biology: Cloning, PCR, RT-PCR, Jumping-PCR, Fushion-PCR, Immuno-PCR, Primer extention, Recombinant protein expression and purification in Escherichia coli, RNA manipulation, cDNA library preparation and construction, Northern blot, Southern blot and Yeast genetic manipulation( Yeast transformation, Recombinant protein expressed in yeast, Preparation the competent cell of yeast…..).
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Cell biology: Microinjection of Xenopus oocyte, Fractionation of cellular organelle, IFA, Flow-cytometry, In situ hybridization and Cell culture.
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Immunology: Immuno-PCR, ELISA, IFA, Western blot, Dot blot, Immunoprecipitation(IP), Polyclonal antiserum manipulation, Supershift analysis, IP-western and antibody purification
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得獎與獎勵
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Poster Presentation Award : The Sixth Symposium of Positive sence RNA virus in Paris, May/28-Jun/2/2001.
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Oral Presentation Award : 19th Annual Meeting of The American Society for Virology in Corolado State University, Jul/8-12/2000.
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中華民國中央研究院生醫所poster比賽得獎助金,獎助出席國際會議May/2000.
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中華民國細胞暨分子生物學會出席國際會議獎助Jun/2001.
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經歷
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服務單位
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系所
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職稱
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起訖時間
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中臺科技大學
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食品科學系
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專任助理教授
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2003/02至今
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中央研究院
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生物醫學研究所
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國科會特約博士後學者
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2002/11至2003/02
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中央研究院
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生物醫學研究所林宜玲實驗室
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博士後學者
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2000/07至2002/10
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中央研究院
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生物醫學研究所
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國科會博士後學者
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1999/09 至2000/06
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國立陽明醫學院
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遺傳學研究所
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研究助理
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1990/08至1991/07
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參與學會
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中華民國細胞暨分子生物學會會員The Chinese Society of Cell and Molecular Biology
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中華民國微生物學會會員The Chinese Society of Microbiology
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研究計畫
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國科會: 研究PKR抗病毒蛋白激脢在日本腦炎病毒感染過程所扮演的角色
計畫編號: 91-2320-B-001-062-
計畫執行起迄: 2002.12.01 至2003.11.30
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國科會: 運動後不同升糖指數碳水化合物補充對人體骨骼肌肉肝醣再合成因子蛋白質表現之影響
計畫編號: 94-2413-H-030-009-
計畫執行起迄: 2005.8.01 至2006.7.31
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教育部: 工業生產菌株的DNA指紋鑑定與資料庫建立
計畫編號: EDU-產-94-008
計畫執行起迄: 2005.8.01 至2005.12.31
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期刊論文
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Yung-Fu Chen, Rung-Ching Chen, Yung-Kuan Chan, and Ren-Hao Pan, You-Cheng Hseu, Elong Lin. (2009) Design of multiplex PCR primers using heuristic algorithm for sequential deletion applications. Computational Biology and Chemistry doi:10.1016/j.compbiolchem.2008.12.003(SCI)
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You-Cheng Hseu, Elong Lin, Jing-Yi Chen, Yi-Ru Liua, Chun-Yin Huang, Fung-Jou Lu, Jiunn-Wang Liao, Ssu-Ching Chen, Hsin-Ling Yang. (2008) Humic acid induces G1 phase arrest and apoptosis in cultured vascular smooth muscle cells. Environmental Toxicology DOI 10.1002/tox(SCI)
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廖淑芬、林一郎、巫錦霖、程一雄。2008。運動後碳水化合物補充對人體骨骼肌代謝基因表現效應-寡核糖核酸微陣列分析。
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Yung-Fu Chen, Rung-Ching Chen, Lin-Yu Tseng, Elong Lin, Yung-Kuan Chan
and Ren-Hao Pan. (2007) NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants. Nucl. Acids. Res. 35, W66–W70 Web Server issue doi:10.1093/nar/gkm305(SCI)
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Ren-Jye Lin, Ching-Len Liao, Elong Lin, and Yi-Ling Lin. (2004) Blocking of the alpha interferon-induced Jak-Stat signaling pathway by Japanese encephalitis virus infection. J. Virology. 78, 9285-9294 (SCI) http://jvi.asm.org/cgi/content/abstract/78/17/9285
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Hsin-Ling Yang, You-Cheng Hseu, Yi-Ting Hseu, Fung-Jou Lu, Elong Lin, and Jim-Shoung Lai. (2004) Humic acid induces apoptosis in human premyelocytic leukemia HL-60 cells. Life science 75, 1817-1831 (SCI)
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Elong Lin, Sue-Wen Lin, and Alan Lin. (2001) The participation of 5S rRNA in the co-translational formation of a eukaryotic 5S ribonucleoprotein complex. Nucl. Acids. Res. 29, 2510-2516 (SCI) http://nar.oupjournals.org/cgi/content/full/29/12/2510
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Elong Lin, Shain-Ren Liu, and Alan M. Lin. (1999) Biochemical analysis of a 5S rRNA- associated sub-particle from trypsinized eukaryotic ribosomes. J. Biochem. 125, 1029-1033 (SCI)
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博士論文
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Elong Lin, Alan M. Lin.(1999) The structure and assembly of the 5S ribosomal ribonucleoprotein complex: studies of eukaryote ribosomal protein L5 interaction and reconstitution with 5S ribosomal RNA. Ph.D. Thesis
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論文發表
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美洲病毒學會年會19th Annual Meeting, The American Society for Virology論文發表(Oral) Inhibition of the double-stranded protein kinase PKR by JEV NS2A protein. July/8-12/2000.
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壁報發表
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歐聯病毒學會第六屆國際正向RNA病毒研討會(Sixth International Symposium on Positive Strand RNA Virus) 壁報發表(Poster) ：Inhibition of Antiviral Protein Kinase PKR by Japanese Encephalitis Viral Proteins. May/28-Jun/2/2001
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第34屆中華民國食品科學技術學會壁報展示論文B-23：以單一引子聚合酶連鎖反應之去氧核醣核酸指紋分析法鑑定酵母菌種之研究.。台北。May/28-Jun/2/2004
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